Antibiotic Resistance of Cholera in Hodeidah, Yemen 2017 versus 2024: Experimental Design
DOI:
https://doi.org/10.59846/ajbas.v4i1.692الملخص
Background: Cholera, caused by Vibrio cholerae (V. cholerae), remains a significant global health threat, particularly in low- and middle-income countries (LMICs) with inadequate sanitation. The increasing emergence of antimicrobial resistance (AMR) in V. cholerae strains complicates treatment, as many commonly used antibiotics (e.g., tetracycline, doxycycline, azithromycin) become less effective. This results in treatment failures, higher morbidity and mortality, and more challenging outbreak control. Regions like Yemen reported high AMR prevalence in cholera strains. Objectives: Therefore , the study aimed to monitor the effectiveness of the antibiotics that used in treatment of cholera infection based on the inhibition zone measurements. Methods: 94 acute watery fecal samples were collected from Diarrhea Treatment Center (DTC) (isolation area) and Center of Tropical Medicine and Infectious Diseases (CTMID) of Authority of Public AL-Thawara Hospital, Hodeidah , Yemen , Outbreak , 2017 and 2024 .The V. cholerae were isolated and diagnosed based on standard microbiological methods. The resistance of antibiotics was monitored based microbiological assay in vitro. Results: The results showed that 34/94 cases (36.17%) of V. cholerae were confirmed. The clinical cholera isolates were sensitive for ciprofloxacin, doxycycline, and tetracycline were stable with the time in 2017 versus 2024 , while the resistance for azithromycin were recorded partially (50 %) in 2017 , and developed into 100 % completely in 2024. Conclusion: The study concluded that ciprofloxacin, followed by doxycycline and tetracycline, is most effective against V. cholerae. It warns against the continued use of azithromycin due to increasing resistance. The findings call for a revision of cholera treatment protocols, emphasizing the need to discontinue azithromycin and to adopt antibiotics based on local resistance patterns, clinical efficacy, and cost-effectiveness.
المراجع
- Ojeda Rodriguez, J. A., Hashmi, M. F., & Kahwaji, C. I. (2024). Vibrio cholerae Infection. In StatPearls. StatPearls Publishing. Copy
- Bertagnolio, S., Dobreva, Z., Centner, C. M., Olaru, I. D., Donà, D., Burzo, S., Huttner, B. D., Chaillon, A., Gebreselassie, N., Wi, T., Hasso-Agopsowicz, M., Allegranzi, B., Sati, H., Ivanovska, V., Kothari, K. U., Balkhy, H. H., Cassini, A., Hamers, R. L., Weezenbeek, K. V., & WHO Research Agenda for AMR in Human Health Collaborators (2024). WHO global research priorities for antimicrobial resistance in human health. The Lancet. Microbe, 5(11), 100902. https://doi.org/10.1016/S2666-5247(24)00134-4
- Schmidt, K., Scholz, H. C., Appelt, S., Michel, J., Jacob, D., & Dupke, S. (2023). Virulence and resistance patterns of Vibrio cholerae non-O1/non-O139 acquired in Germany and other European countries. Frontiers in microbiology, 14, 1282135. https://doi.org/10.3389/fmicb.2023.1282135
- Das, B., Verma, J., Kumar, P., Ghosh, A., & Ramamurthy, T. (2020). Antibiotic resistance in Vibrio cholerae: Understanding the ecology of resistance genes and mechanisms. Vaccine, 38 Suppl 1, A83–A92. https://doi.org/10.1016/j.vaccine.2019.06.031
-Menendez-Gil, P., Veleva, D., Virgo, M., Zhang, J., Ramalhete, R., & Ho, B. T(2024). Modulation of Vibrio cholerae gene expression through conjugative delivery of engineered regulatory small RNAs. Journal of bacteriology, 206(10), e0014224. https://doi.org/10.1128/jb.00142-24
- Dong, C., Zhang, X., Bao, C., Zhu, Y., Zhuang, L., Tan, Z., Qian, H., & Tang, F. (2015). Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine], 49(2), 128–131.
- Gladkikh, A. S., Feranchuk, S. I., Ponomareva, A. S., Bochalgin, N. O., & Mironova, L. V. (2020). Antibiotic resistance in Vibrio cholerae El Tor strains isolated during cholera complications in Siberia and the Far East of Russia. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 78, 104096. https://doi.org/10.1016/j.meegid.2019.104096
- Nateghizad, H., Sajadi, R., Shivaee, A., Shirazi, O., Sharifian, M., Tadi, D. A., & Amini, K. (2023). Resistance of Vibrio cholera to antibiotics that inhibit cell wall synthesis: A systematic review and meta-analysis. Frontiers in pharmacology, 14, 1027277. https://doi.org/10.3389/fphar.2023.1027277
- Gladkikh, A. S., Feranchuk, S. I., Ponomareva, A. S., Bochalgin, N. O., & Mironova, L. V. (2020). Antibiotic resistance in Vibrio cholerae El Tor strains isolated during cholera complications in Siberia and the Far East of Russia. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 78, 104096. https://doi.org/10.1016/j.meegid.2019.104096
- Das, B., Verma, J., Kumar, P., Ghosh, A., & Ramamurthy, T. (2020). Antibiotic resistance in Vibrio cholerae: Understanding the ecology of resistance genes and mechanisms. Vaccine, 38 Suppl 1, A83–A92. https://doi.org/10.1016/j.vaccine.2019.06.031
- Verma, J., Bag, S., Saha, B., Kumar, P., Ghosh, T. S., Dayal, M., Senapati, T., Mehra, S., Dey, P., Desigamani, A., Kumar, D., Rana, P., Kumar, B., Maiti, T. K., Sharma, N. C., Bhadra, R. K., Mutreja, A., Nair, G. B., Ramamurthy, T., & Das, B. (2019). Genomic plasticity associated with antimicrobial resistance in Vibrio cholerae. Proceedings of the National Academy of Sciences of the United States of America, 116(13), 6226–6231. https://doi.org/10.1073/pnas.1900141116
- Orubu, E. S. F., Al-Dheeb, N., Ching, C., Bu Jawdeh, S., Anderson, J., Sheikh, R., Hariri, F., Basaleem, H., & Zaman, M. H. (2021). Assessing Antimicrobial Resistance, Utilization, and Stewardship in Yemen: An Exploratory Mixed-Methods Study. The American journal of tropical medicine and hygiene, 105(5), 1404–1412. https://doi.org/10.4269/ajtmh.21-0101
- Wu, Q., Vaziri, A. Z., Omidi, N., Hassan Kaviar, V., Maleki, A., Khadivar, P., & Kouhsari, E. (2023). Antimicrobial resistance among clinical Vibrio cholerae non-O1/non-O139 isolates: systematic review and meta-analysis. Pathogens and global health, 117(3), 235–244. https://doi.org/10.1080/20477724.2022.2114620
- Kitaoka, M., Miyata, S. T., Unterweger, D., & Pukatzki, S. (2011). Antibiotic resistance mechanisms of Vibrio cholerae. Journal of medical microbiology, 60(Pt 4), 397–407. https://doi.org/10.1099/jmm.0.023051-0
- Chatterjee, P., Kanungo, S., Bhattacharya, S. K., & Dutta, S. (2020). Mapping cholera outbreaks and antibiotic resistant Vibrio cholerae in India: An assessment of existing data and a scoping review of the literature. Vaccine, 38 Suppl 1, A93–A104. https://doi.org/10.1016/j.vaccine.2019.12.003
- Al Kamarany, M. ., & Ghouth, A. S. B. . (2024). The Ice Berge of Cholera in Hodeidah , Yemen : The Contact Exposure. Studies in Medical and Health Sciences, 1(1), 32–35. https://doi.org/10.48185/smhs.v1i1.1169
- Al Sheebani, S.; Al-Kamarany, M.A.; Ghouth, A.B.; Kamal, A.; Alaq, M .
.(2018). Acute renal failure induced by cholera: Outbreak of Hodeidah, Yemen, 2017: European Journal of Pharmaceutical and Medical Research , 5(8), 188–192. https://www.researchgate.net
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الحقوق الفكرية (c) 2025 Maha Al-Asbhi, Ashgan Al-khadery , Hadia Omar , Manal Alomaisi , Mohammed Amood Al Kamarany
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